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Macherey-Nagel
Macherey-Nagel Starter Set Midi for processing NucleoSpin Midi Columns under vacuum
DNA purification from up to 2 mL blood using vacuum filtration1-2 mL whole bloodPrinciple / Procedure NucleoSpin ® Blood L Vacuum is designed for the rapid, medium scale preparation of highly pure genomic DNA from up to 2 mL whole blood using vacuum filtration. The use of vacuum filtration technology eliminates tedious centrifugation steps and manual handling of columns during the process (e.g., discard of collected column flow through). Using the NucleoVac 96 Vacuum Manifold and the Starter Set Midi up to 24 samples can be processed in parallel. The Starter Set Midi consists of SBS format based column holders enabling the use of both manual vacuum manifolds, such as the NucleoVac 96 Vacuum Manifold as well as manifolds of liquid handling platforms. The set includes Column Holder Midi, Wash Plate Midi, and Elution Tube Holder Midi. The purification protocol is based on the lysis of the blood samples using Proteinase K incubation iMeer tonen
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